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m will not be restricted because of the association rate continual for the development from the NagA-substrate sophisticated. These effects (together with the enhanced fee of response of compound 6 relative to compound one) are only consistent with the summary that NagA is proscribed by possibly the formation or breakdown with the tetrahedral intermediate.
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a is definitely the acid dissociation continuous. pH-level profiles that confirmed the loss of catalytic activity at significant and minimal values of pH had been in shape to equation 4, the place K
The structures from the compounds analyzed as substrates for NagA are introduced in Scheme six. The substitution of sulfur for oxygen can be utilized together with challenging and comfortable metals to probe the prospective for steel-ligand interaction during the activation of electrophilic reactions (24, twenty five).
m when the Zn enzyme has the lowest. Equivalent trends while in the number of the catalytic constants had been described previously for the Co, Zn, Mn and Fe reconstitutions of apo-NagA from E. coli
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